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. 2020 Sep 30;13:635–646. doi: 10.2147/JIR.S276619

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© 2020 Guo et al.

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H19 promoted the level of autophagy in PDLCs. (A) Western blot analyses of Beclin-1 and LC3II/LC3I after PDLCs transfected with a vector expressing H19 for 48 h. Histograms show quantification of the band intensities. (B) Images of Beclin-1 and LC3 immunofluorescence staining after PDLCs transfected with a vector expressing H19 for 48 h. Scale bar, 100μm. (C) Western blot analyses of Beclin-1 and LC3II/LC3I in PDLCs treated with TNFα with or without H19 knockdown for 48 h. Histograms show quantification of the band intensities. (D) The flow cytometric results of Beclin-1 in H19 overexpression group and in H19 knockdown group with TNFα stimulation for 48 h. (Analysis of variance *P <0.05; n=9 replicates derived from three donors).