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Primer sequences used in construction of the N. caninum ΔNcGRA6 strain.
| Primer name | Sequence (5′-3′) |
|---|---|
| sgNcGRA6-Fa | GGTGACGCTTGTGGCCTTCAGTTTTAGAGCTAGAAATAGC |
| sgNcGRA6-Rb | AAACAACAATGTCCCTTTGGCA |
| KOc-NcGRA6-F | ATGGCGAACAATAGAACCCTCGCATAAGCTTTACTCGTCGCCAGCAGT |
| KOc-NcGRA6-R | CTATTTTTCCTCCCCGCCGTTTTCGGTCGGAATTTAGGTCGGAAAAGT |
F represented forward.
R represented reverse.
KO represented knockout.
