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Primer sequences used in validation of the N. caninum ΔNcGRA6 strain.
| Primer name | Sequence (5′-3′) |
|---|---|
| P1 | AGTTTCCACTGGCTAGTTTCTTGAA |
| P2 | TCCCACAAGTGCATTCTCCAAAC |
| P3 | GAAGCACACGTTTCAGAGACCA |
| P4 | TAGCTCCAGTGTGTCTGTTCCT |
| NcGRA6-Fa | ATCCGGTTGAATCCGTGGAG |
| NcGRA6-Rb | CTTGTCACGACTGCCTGCTA |
| Actin-F | TGAGAGAGGATACGGTTT |
| Actin-R | GGCAGCGGAAGCGCTCGTT |
F represented forward.
R represented reverse.
