TABLE 3.
Different expressed fold of RHOs and CYPs on IcdP, PYR, and BbF as the sole carbon source in strain IcdP1.
Gene | Function | Induction folda |
|||||
IcdP |
Pyr |
B(b)F |
|||||
recAb | rpoBb | recA | rpoB | recA | rpoB | ||
RHO818 | 2,3-Dihydroxybiphenyl 1,2-dioxygenase | 1.85 | 4.73 | –c | – | – | – |
RHO4741 | Aromatic-ring-hydroxylating dioxygenase, beta subunit | 9.07 | 24.60 | – | – | – | – |
RHO1920 | 3-Phenylpropionate dioxygenase ferredoxin subunit | 8.11 | 22.00 | – | – | – | – |
RHO4740 | 3-Phenylpropionate dioxygenase alpha subunit | – | – | 1.71 | 2.28 | – | – |
RHO4894 | Probable taurine catabolism dioxygenase | – | – | 4.39 | 5.86 | – | – |
865 | Putative cytochrome P450 hydroxylase | – | – | 5.52 | 6.22 | – | – |
1150 | Putative cytochrome P450 hydroxylase | – | – | 2.16 | 2.37 | – | – |
RHO1921 | 2,3-Dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase | – | – | 3.02 | 2.96 | – | – |
RHO4526 | 2,3-Dihydroxybiphenyl 1,2-dioxygenase | 2.16 | 1.97 | 2.32 | 2.29 | – | – |
RHO1892 | 2,3-Dihydroxybiphenyl 1,2-dioxygenase | 4.16 | 11.39 | 2.05 | 3.52 | 2.72 | 3.63 |
RHO1894 | Rieske (2Fe-2S) domain-containing protein | 2.44 | 2.21 | 3.16 | 2.51 | 5.88 | 5.77 |
RHO1917 | 2,3-Dihydroxybiphenyl 1,2-dioxygenase | 2.19 | 6.01 | 2.00 | 3.42 | 1.91 | 2.54 |
RHO1919 | Biphenyl 2,3-dioxygenase | 2.43 | 6.59 | 1.50 | 2.58 | 4.47 | 5.97 |
aOnly those increased for > 1.5-fold in both recA and rpoB as reference genes are identified as significant increase and listed here. brecA or rpoB is used as reference gene for each RHO and CYP450. c –, indicated increase fold < 1.5 of recA or rpoB.