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Localization of amyB mRNA in A. oryzae hyphae cultured in complete media. A. oryzae hyphae were grown in GPY (A,B) or MPY (C,D) media. smFISH was performed without (A,C) or with (B,D) the amyB probe. The images in (E) are magnifications of the region within the white rectangle in (D). The white arrowhead indicates the nucleus located closed to the apex. Note that the amyB fluorescent signals are not seen in the tip region. Scale bars, 20 μm (A–D) and 10 μm (E).
