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Amplification efficiency and melting curve analyses of invA determined in 10-fold serial dilutions of DNA. (A) PCR performed on Salmonella DNA extracted from a pure culture with 1 × 109 CFU/mL. (B) Melting curve of invA (605 bp) in DNA obtained from 10-fold serially diluted (108 to 101 CFU/g) Salmonella spiked soil samples.
