FIGURE 3.

Effects of different concentrations (2.5, 5, and 10 mM) of PMAxx^TM on the amplification of target DNA obtained from the viable and non-viable culture of Salmonella. The data showed that PMAxx^TM efficiently inhibited the amplification of the target DNA obtained only from the non-viable culture of Salmonella. This trial was repeated three times and an average of the data was presented. For obtaining non-viable cells, Salmonella in LB broth was heated at 95°C for 5 min, and a 100 μL plated on XLD agar and incubated overnight at 37°C confirmed that the heat treatment was effective in killing 100% of Salmonella in the broth. Asterisks (^∗∗∗) denote significant difference at P < 0.0001, while ns denotes non-significant difference.