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. 2020 Sep 22;11:581201. doi: 10.3389/fmicb.2020.581201

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Copyright © 2020 Zhang, Khan and Chousalkar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overall viable and non-viable load quantified by PMAxx^TM -based qPCR from Salmonella inoculated soil affected by temperature. (A) Mean log₁₀ viable and non-viable load of Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in soil samples incubated at 5, 25, and 37°C and processed at weekly intervals over 6-week of incubation time period. (B) Mean log₁₀ viable and non-viable load of SE and ST affected by temperature. Samples were treated either with PMAxx^TM or left as control before the extraction of DNA. The DNA copy number was calculated through the standard curve and the data were presented as log₁₀ Salmonella load. Asterisks (^∗∗∗) denote significant difference at P < 0.0001.