FIGURE 5.

MiR-214-3p directly interacted with the 3′UTR of KLF4. (A) Schematic of the putative target sequence for miR-214-3p within KLF4 3′UTR and the mutant in the target sequence. (B) Luciferase activities were evaluated in SK-N-SH cells transfected with KLF4 3′UTR-WT or KLF4 3′UTR-MUT together with miR-NC mimic or miR-214-3p mimic. (C) The enrichment levels of miR-214-3p and KLF4 by anti-Ago2 or anti-IgG antibody in cell lysates of SK-N-SK cells. (D,E) KLF4 protein expression was tested by western blot in 6 MPTP-treated mice and 6 normal controls, and primary neuronal cells after treatment with the indicated concentrations of MPP⁺ for 24 h. *P < 0.05.