Figure 3.

Sequencing analysis of the fecal microbiota obtained from ligatured and no treatment mice 4 weeks after ligation. (A) Diversity of bacterial species as indicated by Chao1 rarefaction measure. Each box plot represents the median, interquartile range, minimum, and maximum values. (B) PCoA of fecal microbiota from ligatured and no treatment mice. (Unweighted distance: R² = 0.187, p = 0.009 < 0.01, Adonis test). (C) Venn analysis of bacterial OTUs composition between two groups (n = 6). (D–E) Proportional taxonomic assignments at the phylum level and genus level in stool samples from two groups. Only genus detected at mean relative abundance top 20 are presented. OTUs, operational taxonomic units; ANOSIM, analyses of similarities; PCOA, principal coordinates analysis; F-4w-E, experimental periodontitis group; F-4w-C, control group.