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. 2020 Oct 5;11:4981. doi: 10.1038/s41467-020-18751-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a HEK293T cells overexpressing GIPR used to determine homologous whole cells ¹²⁵I-GIP membrane binding. Cells treated with or without DA-GIP, washed, and binding using ¹²⁵I-labeled human GIP was measure as CPM in a TopCount NXT gamma counter and plotted as % ¹²⁵I-GIP bound. n = 2 wells/treatment. b, c Comparison of whole cell, membrane, and intracellular GIPR (red, Alexa Fluor 647) content in CHOK1 cells overexpressing GIPR pre-treated with or without DA-GIP for 24 h. b Quantitated GIPR fluorescence (RFU) and c representative image. n = 6 wells/treatment, two-way ANOVA with Sidak’s test for multiple comparisons, ****p ≤ 0.0001. Data are presented as mean values ± SEM. d, e Time course comparison of GIPR internalization upon stimulation with GIP in CHOK1 cells overexpressing GIPR (red, Alexa Fluor 647) pre-treated with or without DA-GIP for 24 h. d, Quantitated membrane GIPR fluorescence (RFU) with background fluorescence subtracted and e, representative images, n = 3 wells/treatment. Data are presented as mean values ± SEM.