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. 2020 Oct 5;10:16495. doi: 10.1038/s41598-020-73457-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of CAP on TMZ induced DNA damage. (A) The immunofluorescence images of glioblastoma U87MG cells antibodies against γ-H2AX for the following variables: untreated control, 180 s CAP (1 treatment), 50 μM TMZ (3 treatments) and combined CAP–TMZ treatments on day 6. (B) The data represents the level of phosphorylated H2AX in each treatment group and is expressed as the mean pixel intensity per cell. These values are calculated by dividing the sum intensity of each image by the total number of cells in the image. A total of 24 fields were analyzed per condition. Error bars indicate the standard error of mean, and the asterisk indicates statistical significance to untreated control conditions unless otherwise noted. Scale bar: 14 μm.