Figure 1.

Activation through CD300e reduces the expression of HLA-DR in all monocyte subsets. Freshly isolated monocytes were plated on 24-well plates uncoated, coated with isotypic IgG (control IgG) or with anti-CD300e monoclonal IgG (UP-H2). At time 0 and after 24 h cells were labelled with anti-CD14, anti-CD16 and anti-HLA-DR antibodies. The expression level of CD14 and CD16 was used to identify 4 cell subsets, namely CD14⁺CD16⁻, CD14⁺CD16⁺, CD14^lowCD16⁺ and CD14^lowCD16⁻. Upper panels: representative cytograms. Lower panels: median fluorescence intensity (MFI) of HLA-DR ± SEM of 4 independent experiments was calculated for each subset. Expression levels of HLA-DR are expressed as n-fold change relative to time 0 (T₀) set as 1 (dotted line). Significance was determined by Student’s t-test. *p ≤ 0.05; **p ≤ 0.01.