Figure 4.

Activation through CD300e affects the synthesis of HLA-II in monocytes. (a–c) mRNA expression of CIITA, HLA-DR α and β chains in monocytes activated or non-activated through anti-CD300e. Monocytes were plated on 24-well plates uncoated, coated with isotype IgG (control IgG) or with anti-CD300e. At the indicated time points, cells were processed for mRNA extraction. mRNA expression was determined by qRT-PCR and data were normalized to an endogenous reference gene (β-actin). Expression levels of treated cells are relative to values at T₀ set as 1 (dotted line). Data are shown as mean ± SEM of 4 independent experiments. (d) HLA-DR synthetic rate in CD300e-activated or non-activated monocytes. Monocytes were plated on 24-well plates uncoated, coated with isotype IgG (control IgG) or with anti-CD300e. After 24 h, cells were processed as detailed in Methods section. Blot refers to a representative of 3 independent experiments. Quantification of HLA-DR was performed by densitometry and expressed as amount relative to untreated cells set as 1 (mean ± SEM). Significance was determined by Student’s t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.