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. 2020 Oct 5;10:16501. doi: 10.1038/s41598-020-73552-9

Figure 5.

Figure 5

Engagement of CD300e dampens the IFN-γ-induced CIITA and HLA-DR expression. (a) mRNA expression of CIITA in monocytes activated or not by anti-CD300e for 6 h and then stimulated for further 6 h with 10 ng/ml IFN-γ. Cells were processed for mRNA extraction. mRNA expression was determined by qRT-PCR and data were normalized to β-actin. Expression levels of treated cells are relative to untreated cells set as 1. Data are shown as mean ± SEM of 4 independent experiments. (b) Surface expression of HLA-DR protein in monocytes activated by anti-CD300e for 16 h and then stimulated for further 8 h with 10 ng/ml IFN-γ. Cell surface level of HLA-DR was evaluated by flow cytometry. MFI of HLA-DR ± SEM of 4 independent experiments was calculated and expressed as n-fold change relative to untreated cells set as 1. Left panels in (a) and (b) describe the experimental setup. Significance was determined by Student’s t-test. **p ≤ 0.01; ***p ≤ 0.001.