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. 2020 Oct 5;6:11. doi: 10.1038/s41514-020-00049-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Redox-titration profile of red blood cells as function of nitrite concentration in a T₁ relaxation and b T₂ relaxation domain. The incubation times were 10 min. The control baseline readings were (T_2o = 149.5, T_1o = 621.3) ms, which is the readings for oxy-Hb without any nitrite exposure. The limit of detection (LOD) is about 0.0005 mM of nitrite concentration (gray bar). The data is represented by box-plot format. The corresponding concentration-dependent c A-ratio, and d T₁–T₂ trajectories of the gradual inversion of Fe²⁺ subpopulation to complete formation of Fe³⁺ population. Time-dependent kinetic profile of ferrous oxidation using nitrite concentrations (500 μM, 4 mM, 8 mM, and 10 mM) in e T₁ relaxation and f T₂ relaxation domain. The corresponding g A-ratio, and h T₁–T₂ trajectories in the magnetic state diagram. Three different samplings were taken from the same donor, and the results were reported as mean ± standard error measurement. For curve fitting, a general function of exp(−Ct)|cos(Ct)| can be used to describe the oscillatory behavior, where C is concentration.