Figure 1.

Concordant and discordant nucleotide base call in sequences analyzed by Gold Standard and by Exatype. Matrices depict the frequencies of nucleotides called by Exatype (vertical axis) and by Standard (horizontal axis). Green highlight implies a concordant base call. Partially discordant base calls (implies mixtures called by one method but not the other) is in yellow highlight. Entirely discordant base calls are in red highlight. Zero is represented by blank cells. International Union of Biochemistry and Molecular Biology ambiguity codes are as follows; R A/G, Y C/T, W A/T, M A/C, K G/T, S G/C, B C/G/T, D A/G/T, H A/C/T, and V A/C/G. Overall, 99.8% concordance was observed for 311,227 bases compared.