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. 2020 Sep 22;8:555378. doi: 10.3389/fcell.2020.555378

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Copyright © 2020 Novoseletskaya, Grigorieva, Nimiritsky, Basalova, Eremichev, Milovskaya, Kulebyakin, Kulebyakina, Rodionov, Omelyanenko and Efimenko.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of adipogenic differentiation of hMSCs cultured on plastic, plastic covered by fibronectin, or laminin, or type I collagen and dECM produced by human fibroblasts or MSCs for 4 days assessed by histological staining with Oil Red. Objective magnification, ×20. The dye was extracted, and Oil Red O staining was quantified by measuring the optical density at 530 nm (lower panel) (n = 4). The quantitative data are represented as median (25%, 75%), ^∗(p value < 0.05), ^∗∗(p value < 0.005), ^*⁣*⁣**(p value < 0.0001) compared to cells cultured on hTERT-MSC dECM and ^▲▲▲(p value < 0.0005) compared to cells cultured on hFibroblast dECM.