Figure 3.

Validation of an LMNB1-Null HeLa Cell Line for Functional Characterization of LMNB1 Variants

(A) Immunoblot analysis of lamin B1 and lamin A/C in parental (LMNB1^+/+) and LMNB1^−/− HeLa cells. GAPDH was used to normalize protein loading.

(B) Immunostaining of lamin B1 (green) and lamin A/C (red) in HeLa cells. DAPI (blue) staining of the nucleus is shown in the merged images.

(C) Transfection of cDNA encoding WT LMNB1 in the LMNB1^−/− HeLa cells greatly increases the abundance of lamin B1 relative to LMNB1^+/+ HeLa cells.

(D) Expression of WT and variant-encoding cDNA in the LMNB1^−/− HeLa cells followed by immunoblot analysis of whole-cell lysates.

(E) Quantification of the abundance of lamin B1 (relative to GAPDH) by densitometry in transfected HeLa cells (n = 4); ^∗p ≤ 0.05.