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. 2020 Sep 9;107(4):753–762. doi: 10.1016/j.ajhg.2020.08.015

Figure 4.

Figure 4

Introduction of Variant-Bearing LMNB1 cDNAs Disrupts Nuclear Envelope Integrity

(A) Immunostaining of LMNB1−/− HeLa cells transfected with WT and variant-bearing LMNB1 cDNAs (lamin B1, green; lamin A/C, red; DAPI, blue). The number of transfected cells with abnormal nuclear envelope morphology (versus the total cell quantified across at least four different fields from three independent staining experiments) is denoted in the middle panels. Scale bar represents 10 μm.

(B) Representative nuclear phenotypes observed in the transfected HeLa cells that were scored (the condensed phenotype was taken from the p.Ala152Gly cells and the bi-/multi-lobed phenotype was taken from the p.Lys33Glu cells); scale bar represents 10 μm.

(C) Quantification of the percentage of nuclei with a given phenotype from at least 100 cells scored across the three independent staining experiments; statistical analysis done with a Dunnett’s test; ∗∗p ≤ 0.01, ∗∗∗∗p ≤ 0.0001.