FIGURE 2.

Tissue distribution of Spata1 mRNA and protein. A, Total RNA was extracted from indicated mouse tissues and the distribution of Spata1 transcripts was analyzed by RT-PCR. Notice that Spata1 mRNA was highly expressed in the testis and kidney, and it was also expressed in other tissues at a low level. B, Generation of a specific anti-SPATA1 polyclonal antibody. COS-1 cells were transfected with pEGFP-N2 and SPATA1/pEGFP-N2 plasmids respectively, and the protein lysates were subjected to Western blotting with an anti-GFP antibody (a) or an anti-SPATA1 antibody (b). C, Expression profile of the SPATA1 protein was examined in the indicated tissues by Western blotting. The antibody recognized the predicted 52 kDa SPATA1 in the heart and testis; a smaller protein in the brain and multiple proteins in the kidney, presumably representing other SPATA1 isoforms