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. Author manuscript; available in PMC: 2020 Nov 27.
Published in final edited form as: Nature. 2020 May 27;583(7814):122–126. doi: 10.1038/s41586-020-2337-2

Extended data 1 -. The effects of LbNOX expression in primary hepatocytes.

Extended data 1 -

(b) Dose-dependent adenovirus-mediated expression of LbNOX in primary hepatocytes at 24 hours. Representative western from two independent experiments (b) Effect of LbNOX on free cytosolic NADH/NAD+ as measured by peredox with increasing alcohol concentrations. (c) Effect on basal or antimycin+rotenone insensitive respiration with LbNOX or mitoLbNOX. n=3. (d) whole cell NAD+; (e) NADH; (f) NADH/NAD+, and (g) NADH+NAD+. n=6 independent hepatocyte isolations. Nominal p values were determined using paired two-sided Student’sT tests between hepatocyte isolations (c-g), or unpaired two-sided Student’s T test for peredox experiments (b).