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. Author manuscript; available in PMC: 2020 Nov 27.
Published in final edited form as: Nature. 2020 May 27;583(7814):122–126. doi: 10.1038/s41586-020-2337-2

Extended data 2 -. Nicotinamide riboside and LbNOX have distinct effects on pyridine dinucleotide pool sizes and redox ratios.

Extended data 2 -

Relative total cellular (a) NAD+, (b) NADH, (c) NADH/NAD+, secreted (d) lactate/pyruvate, (e) β−hydroxybutyrate/Acetoacetate (βHB/AcAc), and (f) αHB in primary hepatocytes with or without NR supplementation and LbNOX. (g) Effect of pyruvate, LbNOX expression, or NR on the inhibition of Hela cell proliferation and total NAD+ levels (h) by piericidin 4 days after seeding. Data are mean ± s.e.m from n=7 (a-c) or 10 (d-f) independent hepatocyte isolation, or 3 independent Hela cell experiments (g-h). Nominal p values were determined using paired (between hepatocyte isolations, a-f) or unpaired (Hela cells, g-h) two-sided Student’s T tests.