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. 2020 Jul 6;184(2):1024–1041. doi: 10.1104/pp.20.00297

Figure 6.

Figure 6.

PSP231 directly interacts with GhRBPL1 to form a complex. A, Yeast two-hybrid assay of the interaction between PSP231 and GhRBPL1. Yeast transformants containing pGBKT7-PSP231 or pGADT7-GhRBPL1 were cultivated on quadruple- (QDO) or double-dropout (DDO) nutritional selection medium. Negative controls were pGBKT7 and pGADT7 empty vectors, and positive controls were P53 and SV40 large T antigen. B, BiFC assay of the interaction between PSP231 and GhRBPL1. PSP231-LUCc and GhRBPL1-LUCn were coexpressed in leaves of N. benthamiana, using nLUC and cLUC as negative controls (see “Materials and Methods”). C, In vitro pull-down assay of the interaction between PSP231 and GhRBPL1. MBP-GhRBPL1 fusion protein was pulled down with His-PSP231 fusion protein and detected by immunoblotting with anti-MBP antibody, using MBP protein as a control. As a negative control, no His-PSP231 protein was added in the experiment.