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. 2020 Jul 6;184(2):1024–1041. doi: 10.1104/pp.20.00297

Figure 7.

Figure 7.

GhRBPL1 specifically recognizes GhWRKY15 mRNA to form a stable RNA-protein complex. A, Schematic diagram of the positions of putative GhRBPL1 binding motifs (UG-rich motifs U1–U6) in the GhWRKY15 mRNA sequence. B, RNA pull-down assay of the interaction between GhRBPL1 and GhWRKY15 mRNA. MBP-GhRBPL1 fusion protein was pulled down with GhWRKY15 mRNA and detected by immunoblotting with anti-MBP antibody, using MBP protein as a control. As a negative control, no mRNA of GhWRKY15 was added in the experiment.