Figure 6.
Engineering of trilobatin and phloridzin production in N. benthamiana. N. benthamiana leaves were infiltrated with Agrobacterium tumefaciens suspensions containing pHEX2_PGT2, pHEX2_PGT1, or the negative control pHEX2_GUS (each in combination with pHEX2_MdMyb10, pHEX2_MdCHS, and pHEX2_MdDBR + pBIN61-p19). Production of trilobatin and phloridzin were analyzed by Dionex-HPLC analysis (Thermo Fisher Scientific) at 7-d post-infiltration. Experiments were performed in triplicate and a single representative trace is shown. A, pHEX2_PGT2. B, Trilobatin (T) standard. C, pHEX2_PGT1. D, Phloridzin (P) standard. E, Negative control pHEX2_GUS.