Table 3. Substrate specificity of recombinant PGT2 cloned from M. toringoides.
The products of reactions using UDP-glucoside as the sugar donor and the 12 substrates shown were determined by LC-MS/MS. Conversion percent is the amount of product formed relative to the conversion of phloretin to trilobatin, which was set at 100%. All reactions were performed in triplicate. Nd, No products detected.
| Substrate | Product | Conversion |
|---|---|---|
| % | ||
| Phloretin | Trilobatin | 100.0 ± 6.1 |
| 3-OH Phloretin | Sieboldin | 58.7 ± 2.3 |
| Quercetin | Quercetin 3-o-glucoside | 9.1 ± 0.1 |
| Phloridzin | Nd | 0 |
| Trilobatin | Nd | 0 |
| Sieboldin | Nd | 0 |
| Naringenin | Nd | 0 |
| Cyanidin | Nd | 0 |
| Caffeic acid | Nd | 0 |
| 4-Coumaric acid | Nd | 0 |
| Neohesperidin | Nd | 0 |
| Chlorogenic acid | Nd | 0 |
| Boiled protein | Nd | 0 |