Figure 8.

Auto-immune response was triggered on the CsACD2-RNAi transgenic Duncan grapefruit. A, Chlorosis and spontaneous cell death were observed on the CsACD2-RNAi positive transgenic citrus. Top, erGFP fluorescence screening on transgenic CsACD2-RNAi plant. Bottom, photos of transgenic plants in the bright field. Negative-control plant is wild-type Duncan plant which did not show erGFP fluorescence signal. B, RT-qPCR analysis of defense-related genes. Expression of FRK1, GST1, WRKY22, WRKY29, PR1, PR3, and PR5 in the CsACD2-RNAi transgenic citrus was compared with the wild-type Duncan grapefruit. The housekeeping gene encoding glyceraldehyde-3-phosphate dehydrogenase-C (GAPDH-C) was used as an endogenous control. Error bars indicate se (n = 4). Lowercase letters represent significant differences in different types of samples by one-way ANOVA followed by lsd post hoc test (P < 0.05). All experiments were repeated three times with the similar results.