Figure 1.

The TCP4 TF physically interacts with AtWRI1. A, TCP4 shows physical interaction with AtWRI1 variants in the Y2H assay. Yeast growth on either stringent selective (-Leu/-Trp/-His [-LTH]) or permissive (-Leu/-Trp [-LT]) medium is shown. Empty vector (EV) was used as a negative control. B, AtWRI1 interacts with the TCP4 TF in planta. A BiFC assay was performed to validate the interaction between AtWRI1 and TCP4. N. benthamiana epidermal cells were transiently cotransformed with plasmids encoding nYFP-AtWRI1 and cYFP-TCP4 as indicated. Bars = 20 μm.