Figure 2.

Fertility of cals5-2 is restored under low light intensity. A, cals5-2 showed severe male sterility under normal conditions. Pollen production was abnormal in cals5-2 anther. B, The fertility of cals5-2 was restored under low light intensity. Pollen grains in cals5-2 were produced under low light intensity. Bars = 20 μm. C, Pollen growth, from tetrad to mature pollen, was delayed by 1 d under low light intensity (n = 12). D, Diameter of microspores at different developmental stages. Twenty microspores or pollen grains were used to measure the diameter at each developmental stage, respectively. One-way ANOVA was performed to compare the diameter of microspores (*P < 0.05 and **P < 0.001; ns, not significant [P > 0.05]). Error bars represent the sd of pollen diameters. The diameter of microspores at 48 and 96 h was significantly smaller under low light intensity compared to normal conditions.