Figure 2.

Gene structure and expression of P. andersonii PanLYK3. A, Structure of the PanLYK3 gene model and encoded proteins. PanLYK3 possesses two transcriptional start sites resulting in two protein variants, which differ in the extracellular region containing the LysM domains and are encoded by exon 1. The red cross indicates a third upstream copy of exon 1 lost in P. andersonii but maintained in other Parasponia and Trema species. B, Expression profile of PanNFP1, PanNFP2, PanLYK1, and PanLYK3 in different plant tissues. Expression is given in DESeq2-normalized read counts; error bars represent se of biological replicates. Circles represent individual expression levels. The analysis is based on data presented by van Velzen et al. (2018). C, Relative expression of the PanLYK3.1 and PanLYK3.2 transcriptional variants based on RNA sequencing reads splicing into the second exon. Data are represented as means ± se (n = 3). The analysis is based on data presented by van Velzen et al. (2018).