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. 2020 Jul 15;184(2):1004–1023. doi: 10.1104/pp.19.01420

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — P. andersonii PanLYK3 is essential for chitin-triggered immunity responses in roots. A and B, Production of ROS measured upon treatment with 100 μm CO7 (filled circles) or water (open circles) with EV Control 1 plants (black), Panlyk3 line a2 (red), and Panlyk3 line e1 (orange; A) and EV Control 1 plants (black), Panlyk3.1 line a4 (blue), Panlyk3.2 line a1 (red), and Panlyk3.2 line c1 (orange; B). For A and B, data are averages of at least three independent biological replicates ± se. Luminescence is measured in relative light units (RLU). C and D, Phosphorylation of MAPK analyzed by immunoblot using an anti-p44/42 MAPK antibody upon treatment with 100 μm CO7 (top). Equal loading was confirmed by Coomassie Brilliant Blue (CBB) staining (bottom). Results shown are representative of three independent experiments. C, MAPK phosphorylation in root pieces of EV Control 1, Panlyk3 line a2, and Panlyk3 line e1. D, MAPK phosphorylation in root pieces of EV Control 1, Panlyk3.1 line a4, Panlyk3.2 line a1, and Panlyk3.2 line c1.