flg22-induced aROS is increased by AtOXR2 overexpression. A, Time-course kinetics of flg22-triggered aROS burst in wild-type (Wt) Col-0 plants, AtOXR2-overexpressing lines A and B, and the mutant oxr2.2. The aROS burst profiles of 24 leaf discs per genotype were averaged. Results are expressed as means ± sd. B, Quantitation of the area under the curve of flg22-induced ROS performed in the previously mentioned genotypes. Percentages were calculated relative to the wild-type levels. Values represent averages of independent experiments expressed as means ± sd. Asterisks indicate significant differences with regard to the wild type by lsd Fisher’s test (*P < 0.01). RLU, Relative light units.