Table 2.
Cryo-EM data collection and processing.
| Data collection and processing | EWI-FΔIg1-5–CD9–4C8 EMDB-11053 |
|---|---|
| Microscope | Talos Arctica |
| Camera | Gatan K2 Summit + GIF |
| Magnification | 130,000 |
| Voltage (kV) | 200 |
| Exposure time frame/total (s) | 0.2/7.2 |
| Number of frames | 36 |
| Electron exposure (e−/Å2) | 52.0 |
| Defocus range (μm) | −0.8 to −3.0 |
| Pixel size (Å) | 1.029 |
| Symmetry Imposed | C1 |
| Micrographs (no.) | 10,724 |
| Initial particle images (no.) | 1,148,718 |
| Final particles images (no.) | 354,272 |
| Map Resolution (Å) | 8.57 |
| 0.143 FSC threshold | |
| Map resolution range (Å) | 7.9–17.4 |