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. 2020 Sep 23;3(12):e202000824. doi: 10.26508/lsa.202000824

Figure 1. Meiotic Mps2 binds to Csm4 and Mps3.

Figure 1.

(A) Schematic diagram showing the composition and location of c-LINC and t-LINC complexes in budding yeast. Domain organization of Csm4, Mps2, and Mps3 is shown at the bottom. (B) List of representative proteins copurified with TAP-Csm4. (C) Reciprocal immunoprecipitation showing Mps2-Csm4 interaction. The level of Pgk1 serves as a negative control for affinity purification. (D) Reciprocal immunoprecipitation showing Mps2-Mps3 interaction. Note that the anti-V5 antibody also recognizes TAP-Mps2. At least two biological replicates were performed. (E) Localization of Mps2 and Csm4 at prophase I. Three continuous optical sections are shown. Arrows point to the putative localization of Mps2 to the spindle pole body. Note that both Csm4 and Mps2 localize to the nuclear periphery. Dashed oval shows the overall cell shape. Red, Mps2-mApple; green, Csm4-GFP. CC, coiled coil; INM, inner nuclear membrane; ONM, outer nuclear membrane; TM, transmembrane domain.