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. 2020 Sep 23;3(12):e202000824. doi: 10.26508/lsa.202000824

Figure 6. Reconstitution of t-LINC complex in vegetative yeast cells.

Figure 6.

(A) Induction of t-LINC complex formation in mitosis with ectopic Csm4. Time-lapse fluorescence microscopy showing the localization of Mps3-GFP (green). Tub4-mApple (red) marks the spindle pole body (SPB). Arrows pointing to the Mps3 patch formed in the developing daughter cell when Csm4 was produced. Projected images from 12 z-sections are shown. Time zero refers to the onset of SPB separation. Schematic diagrams of c-LINC and t-LINC complexes are shown to the right. (B) Reconstituted t-LINC complex tethers telomeres. Time-lapse fluorescence microscopy was performed as above. Rap1-GFP (green) marks the telomeres; Tub4-mApple (red) marks the SPB. Projected images from 12 z-sections are shown. Time zero refers to the onset of SPB separation. Note that in the presence of Csm4, Rap1-GFP formed a patch in the developing daughter cell. INM, inner nuclear membrane; M, mother cell; ONM, outer nuclear membrane.