Figure S4. Mps2 is required for bridging Csm4 and Mps3 to reconstitute the t-LINC complex.

(A) Cell growth assay. 10-fold diluted yeast cells were spotted onto raffinose or galactose medium. Note that in the absence of Mps2 and Pom152, Csm4 is no longer toxic in vegetative yeast cells. (B) Mps2 is required for forming the Mps3 patch in daughter cells. A similar experimental procedure was carried out as shown in Fig S3A. Live-cell fluorescence microscopy was performed as in Fig S3C. Note that Mps3-GFP no longer forms a patch in the daughter cell when Mps2 is absent. Red, Tub4-mApple; green, Mps3-GFP. M, mother cell.