Figure 4.

Conservation in the gliotoxin BGC correlates with conserved production of gliotoxin analogs in A. fumigatus and nonpathogenic close relatives. Microsynteny analysis reveals a high degree of conservation in the BGC encoding gliotoxin across all isolates. The known gliotoxin gene cluster boundary is indicated above the A. fumigatus Af293 BGC. Black and white squares correspond to evidence or absence of evidence of secondary metabolite production, respectively. Genes are drawn as arrows with orientation indicated by the direction of the arrow. Gene function is indicated by gene color. Gray boxes between gene clusters indicate BLAST-based similarity of nucleotide sequences defined as being at least 100 bp in length, share at least 30% sequence similarity, and have an expectation value threshold of 0.01. Genus and species names are written using the following abbreviations: Afum, A. fumigatus; Aoer, A. oerlinghausenensis; Afis, A. fischeri. Below each genus and species abbreviation is the cluster family each BGC belongs to and their cluster number.