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. 2020 Oct 5;8(2):e000905. doi: 10.1136/jitc-2020-000905

Figure 6.

Figure 6

Combination of pep-20-D12 treatment with IR synergistically delays tumor growth. C57BL/6 mice were transplanted with 1×106 MC38 cells on the right flank, and tumors were allowed to grow to ~100 mm3 before treatment with IR. (A, B) Tumors locally received one 20 Gy dose IR, and 2 mg/kg of pep-20-D12 was daily i.p. injected from the same day of IR for 2 weeks. Tumor growth was monitored after IR (n=7). (C, F) Infiltrating immune cell subsets in tumors were analyzed after treatment. The percentages of intratumoral macrophages (CD45+CD11b+F480+) (C, D) and monocyte-derived MDSCs (CD45+CD11b+Ly6C+Ly6G-) (E, F) in total tumor tissues were evaluated by flow cytometry (n=5 to 6). Data are represented as means±SEM, Statistical significance was determined by one way analysis of variance. *p<0.05; **p<0.01; ***p<0.001. i.p.intraperitoneally; IR, irradiation; MDSC, myeloid-derived suppressor cells; n.s., no significance; s.c., subcutaneously.