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. 2020 Aug 17;152(10):e202012631. doi: 10.1085/jgp.202012631

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© 2020 Neginskaya et al.

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Figure 8. — The effect of PEGs with different molecular weights on the shrinkage of PT-induced mitoplasts. Mitochondrial swelling was induced by microperfusion with 2 mM Ca²⁺. Shrinkage was performed as described in Fig. 5. PEG 400 did not lead to mitoplast shrinkage, indicating permeability of the inner mitochondrial membrane to the molecules of 400 D molecular weight after PT activation with calcium. PEG 2000 (2,000 D) led to mitoplast shrinkage. The experiment confirmed the presence of the classical PTP channel with molecular cutoff ∼1.5 kD. V, volume.