Figure S4.
Transcriptional TEFF profile of T cell clones is linked to memory generation capacity. scRNA-seq and single-cell barcode sequencing were performed on barcode-labeled TEFF cells responding to skin vaccination. The TEFF cells were grouped into 14 transcriptionally distinct MCs. (A) Relative contribution of each mouse to the 14 MCs. (B) Number of cells assigned to each MC. Colors indicate the transcriptional TEFF subset individual MCs were assigned to; see Fig. 4 C for the definition of the identified TEFF subsets. (C) Pie chart depicting the relative frequency of the four identified TEFF states. (D) Number of TEFF cells observed for each reliably detected clone. (E) Comparison of TCIRCM clone size to the relative contribution of clones to the indicated TEFF states (i.e., TCIRCM-like, TRM-like MP, TE, or Int). Spearman correlation r (when significant) and Spearman correlation P value are depicted. Dots represent individual clones. Clones that were not detected in the TCIRCM compartment were excluded. Red line represents the linear regression line. (F) Analysis as depicted in Fig. 6 B, but here depicting mean effector phase TE and Int output of large and small TCIRCM and TRM clones. (G) Comparison of the absolute production of TRM-like MP (top) and TCIRCM-like MP (bottom) of clones to their production of mature TRM. Black line indicates linear regression line with 95% confidence interval in gray.