Figure S2.

Increased CD32a^H-associated presentation and cross-presentation is codependent on FcRn and CD32a. (a–c) CD32a (a), mFcRn (b), and classical FcγR (c) surface expression by primary splenic CD11c⁺MCHII⁺ DCs from CD32a^R-Tg (R; red), CD32a^H-Tg (H; gray), FcγR^−/− (black), and mFcRn^−/− (white) mice (n = 3). Bar graphs representing the average MFI ± SEM, and representative histograms (right for a and b) are shown. The Fcgrt^−/− mice (n = 3) served as negative and positive controls for mFcRn and classical FcγR staining, respectively. (d–f) H2-K^b (d), hFcRn (e), and CD32a (f) surface expression on CD32a^R- or CD32a^H- or vector control plasmid–transfected HEK 293T^H2-Kb cells. For panels d–f, bar graphs display average MFI ± SEM in triplicate. (g) IFN-γ production by CD8⁺ OT-I T cells after 48 h of co-culture with HEK 293T^H2-Kb cells expressing CD32a^R or CD32a^H and loaded with hIgG1^WT ICs or hIgG1^IHH ICs. Data are representative of two or three (a–g) independent experiments with individual points representing triplicate technical replicates. H, CD32a^H; R, CD32a^R; Vector, control vector. All data were analyzed by two-way ANOVA followed by the two-stage linear step-up procedure of Benjamin, Krieger, and Yekutieli with FDR controlled at <0.05. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001.