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. 2020 Aug 4;217(10):e20191515. doi: 10.1084/jem.20191515

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© 2020 Varga et al.

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Figure 3. — NOTCH3 signaling is up-regulated in the tumors of Trp53^ΔIECAkt^E17K mice. (a) Cross-species comparison of the differentially regulated genes identified by RNA sequencing to human gene sets related to NOTCH signaling (Guinney et al., 2015) by GSEA. Positive NES indicates a positive enrichment. n = 4 per genotype. (b) Relative gene expression levels of Notch1–4 in the tumors of Trp53^ΔIEC and Trp53^ΔIECAkt^E17K mice as determined by quantitative RT-PCR (qRT-PCR). Data are mean ± SD, n ≥ 4 per genotype; *, P < 0.05 by t test. (c) Representative images of N3ICD immunohistochemistry (IHC) on sections from AOM-induced colon tumors in Trp53^ΔIEC and Trp53^ΔIECAkt^E17K mice. Scale bars = 200 µm.