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. 2020 Aug 4;217(10):e20191515. doi: 10.1084/jem.20191515

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© 2020 Varga et al.

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Figure S2. — Analysis of gene expression changes in response to Notch3 silencing in Trp53^−/−Akt^E17K organoids. (a) Principal component analysis (PCA) of the RNA-sequencing results of Trp53^−/−Akt^E17KshCo and Trp53^−/−Akt^E17KshNotch3 organoids cultured in the presence of doxycycline for 10 d. (b) Significant gene expression changes induced by Notch3 knockdown for selected genes as determined by RNA sequencing. Changes in the gene expression in Trp53^−/−Akt^E17KshNotch3 organoids were determined relative to control Trp53^−/−Akt^E17KshCo organoids. Experiment was performed in duplicate; P_adj < 0.05. (c) Heatmap and cluster analysis showing expression z-scores of NOTCH3 target genes in AOM-treated CMS2-like Trp53^ΔIEC and CMS4-like Trp53^ΔIECAkt^E17K tumors. n = 3 mice per genotype.