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. 2020 Aug 4;217(10):e20191515. doi: 10.1084/jem.20191515

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© 2020 Varga et al.

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Figure S3. — Notch3 silencing in s.c. and i.v. tumors. (a) Relative mRNA expression level of the indicated genes in s.c. tumors of NBSGW mice transplanted with Trp53^−/−Akt^E17KshCo or Trp53^−/−Akt^E17KshNotch3 organoids day 10 after the start of doxycycline administration by qRT-PCR. Data are mean ± SD; n ≥ 3 per group; *, P < 0.05; **, P < 0.01 by one-way ANOVA followed by Dunnett’s multiple comparison test. Data are shown for a dataset of n = 1 experiment. (b) Schematic depiction of the strategy used for i.v. injection of Trp53^−/−Akt^E17KshCo or Trp53^−/−Akt^E17KshNotch3 organoids and doxycycline administration. (c) Representative images of tGFP immunohistochemistry (IHC) on sections from lung tumors of NBSGW mice i.v. injected with Trp53^−/−Akt^E17KshCo or Trp53^−/−Akt^E17KshNotch3 organoids 6 wk after the start of doxycycline administration. Scale bars = 5 mm (upper row); 200 µm (lower row).