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. 2020 Jul 22;217(10):e20191761. doi: 10.1084/jem.20191761

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© 2020 Poholek et al.

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Figure 3. — STAT3 regulates proliferation, but not apoptosis, in Th17 cells. (A and B) Live CD4⁺YFP⁺ cells were sorted from dLNs on day 10 after immunization and analyzed by RNA sequencing. (A) Heatmap of cell cycle–associated genes differentially expressed in Th17^ctrl versus Th17^ΔSTAT3 cells. (B) GSEA of cell cycle stage. NES, normalized enrichment score. (C) Cell cycle stage analyzed by flow cytometry on day 10 after immunization, following BrdU injection in vivo. (D) Apoptosis analyzed by flow cytometry for activated caspase 3 on indicated days after immunization. Data points show values for individual mice with mean ± SD, pooled from two or three experiments. **, P < 0.01; ****, P < 0.0001, one-way ANOVA (C) and Student’s t test (D).