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. 2020 Sep 21;10:570113. doi: 10.3389/fonc.2020.570113

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Copyright © 2020 Wang, Zhai, Zhang, Han, Zhang, Yao and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TANs and CAFs in gastric cancer tissues. TANs and CAFs were detected in the tumor stroma of all these EGC patients by H&E staining and IHC. (A) Both the high-TAN and high-CAF groups: more than 10 TANs per HPF (black arrow) were detected, and CAFs (yellow arrow) accounted for more than 50% of tumor stroma. (B) The high-TAN group with low CAFs: more than 10 TANs per HPF (black arrow) were detected, and CAFs accounted for <50% of tumor stroma. (C) The low-TAN group with high CAFs: <10 TANs per HPF were detected, and CAFs (yellow arrow) accounted for more than 50% of tumor stroma. (D) The low-TAN group with low CAFs: <10 TANs per HPF were detected, and CAFs accounted for <50% of tumor stroma. (A1, B1, C1, D1) TANs using MPO staining. (A2, B2, C2, D2) CAFs using α-SMA staining.