Table 2. Overrepresented GO terms for gene sets of interest.

Gene sets	Category	p-value	#DE genes	#Genes in category	GO term	#Genes down-regulated	#Genes up-regulated
1: female specific transcripts altered in both the 1 ng/L EE2 and 100 µg/L Atrazine treatments	GO:0006605	0.0033	6	96	Protein targeting	6
	GO:0042254	0.00757	6	127	Ribosome biogenesisa	6
	GO:0007005	0.00975	5	89	Mitochondrion organization	5
	GO:0006913	0.0149	4	63	Nucleocytoplasmic transport	4
	GO:0006810	0.038	29	1,352	Transportb	16	13
	GO:0044281	0.0473	14	564	Small molecule metabolic processc	12	2
2: transcripts altered in males in both the 1 and 100 µg/L Atrazine treatments	GO:0006412	0.00131	14	282	Translation	14
	GO:0044281	0.00329	23	564	Small molecule metabolic processc	21	2
	GO:0006520	0.00336	9	133	Cellular amino acid metabolic process	8	1
	GO:0055085	0.00382	22	495	Transmembrane transportd	15	7
	GO:0009058	0.0262	54	1,916	Biosynthetic process
	GO:0042254	0.0396	6	127	Ribosome biogenesisa	6
	GO:0008150	0.0464	203	8,605	Biological process
3: transcripts altered in females in both the 1 and 100 µg/L Atrazine treatments	GO:0006810	0.00196	18	1,352	Transportb	1	17
	GO:0007267	0.00211	6	249	Cell–Cell signaling		6
	GO:0055085	0.00708	9	495	Transmembrane transportd		9
	GO:0007010	0.0189	7	370	Cytoskeleton organization		7
	GO:0007165	0.026	19	1,709	Signal transduction	1	18
	GO:0002376	0.0271	7	428	Immune system process		7
	GO:0051604	0.0345	2	42	Protein maturation		2

Note:

Gene ontology analysis of differentially expressed (DE) annotated transcripts identified by gene set 1, 2, or 3. Only GO terms that were significantly overrepresented (p-value < 0.05) are presented. Corresponding letters identify common GO terms between gene sets.