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. 2007 Jul 9;17(10):1104–1119. doi: 10.1093/glycob/cwm073

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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

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Fig. 5 — MALDI-TOF-MS and MS/MS of glycans released by endoglycoceramidase from egg glycosphingolipid fraction 6. Oligosaccharides were released from fraction 6 egg glycosphingolipids by treatment with endoglycoceramidase and analyzed by MALDI-TOF-MS (A) and MS/MS (B). (A) MALDI-MS spectrum of glycans liberated from fraction 6 glycosphingolipids. Monoisotopic masses of pseudomolecular ions ([M+Na]⁺) and deduced monosaccharide compositions are assigned. H: hexose; N: N-acetylhexosamine; dH: deoxyhexose (Fucose). Inset in (A): MALDI-TOF-MS of oligosaccharides released from fraction 6 egg glycosphingolipids after subsequent incubation with HF to release fucose residues. (B) Sodiated pseudomolecular ions of the glycan species m/z 2005.6 [M+Na]⁺ with a composition of Hex₁HexNAc₆dHex₄, obtained by endoglycoceramidase treatment of fraction 6 egg glycosphingolipids, were analyzed by MALDI-TOF-MS/MS in the LID mode. Composition is given in symbols according to the Consortium for Functional Glycomics (see Table I for details); light grey square: N-acetylgalactosamine; dark square: N-acetylglucosamine; circle: glucose; triangle: deoxyhexose (fucose). Fragments in the structure are assigned using the nomenclature of Domon and Costello (Domon and Costello 1988). For the sake of clarity only the composition and not the putative origin of each fragment is assigned in the spectrum. H: hexose; N: N-acetylhexose; dH: deoxyhexose. Protonated ions are marked with an asterisk. Signals resulting from single cleavages are specified. Fragments verifying the presence of a terminal HexNAc₂dHex₂ unit are presented in bold-type.