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. 2007 Jul 9;17(10):1104–1119. doi: 10.1093/glycob/cwm073

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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

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Fig. 7 — Binding characteristics of wild-type L-SIGN and L-SIGN mutants. The binding of K562 cells stably expressing wild-type L-SIGN, as well as K562 cells stably expressing the L-SIGN mutants S363V and S363G, to S. mansoni egg glycosphingolipids and SEA is shown using cell adhesion assays. Total egg glycosphingolipids (Egg), defucosylated egg glycosphingolipids (Egg HF) and fraction 6 egg glycosphingolipids (Egg 6) as well as SEA, endo H-treated SEA (SEA endo H) and defucosylated SEA (SEA HF) were coated. Le^A-PAA (Le^A) and Le^X-PAA (Le^X) were used as controls. The experiment shown is a representative of three independent experiments, all performed in triplicate, with error bars indicating standard deviation.